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| Medina Armijo,Cristy A.; Moraga Mamani,Ruben. |
| ABSTRACT Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the abundance of prokariotic community from the sediments of different sites and along a temporary-space scale from Salar de Lagunilla, a high-altitude athalassohaline wetland in the Chilean Altiplano. Six different taxonomic groups were studied: alpha, beta, gamma proteobacterias, sulfate-reducing of the delta subclass of Proteobacteria, Cytophaga-flavobacteria and the domain Archaea. The analyses showed the Archaea domain and Cytophaga-flavobacteria group (33.6 % - 20.2 %, respectively) as the dominant group, whereas the groups with minor abundance corresponded to alpha and Beta-proteobacteria (10.7-15.5 %). The prokariotic communities in... |
| Tipo: Journal article |
Palavras-chave: Fluorescence in situ hybridization; Salt flats; Andean Altiplano. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382016000200181 |
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| Traldi,Josiane B.; Lui,Roberto L.; Martinez,Juliana de F.; Vicari,Marcelo R.; Nogaroto,Viviane; Moreira Filho,Orlando; Blanco,Daniel R.. |
| ABSTRACT The transposable elements (TE) have been widely applied as physical chromosome markers. However, in Loricariidae there are few physical mapping analyses of these elements. Considering the importance of transposable elements for chromosomal evolution and genome organization, this study conducted the physical chromosome mapping of retroelements (RTEs) Rex1, Rex3 and Rex6 in seven species of the genus Harttia and four species of the genus Hypostomus, aiming to better understand the organization and dynamics of genomes of Loricariidae species. The results showed an intense accumulation of RTEs Rex1, Rex3 and Rex6 and dispersed distribution in heterochromatic and euchromatic regions in the genomes of the species studied here. The presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Armored catfish; Fluorescence in situ hybridization; Hypostominae; Loricariinae; Transposable elements. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200214 |
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| Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira. |
| ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum sp.; Molecular cytogenetics; Fluorescence in situ hybridization; Aluminum tolerance; Toxicity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102 |
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| Linardi,C.C.G.; Martinez,G.; Velloso,E.D.R.P.; Leal,A.M.; Kumeda,C.A.; Buccheri,V.; Azevedo,R.S.; Peliçario,L.M.; Dorlhiac-Llacer,P.. |
| Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Multiple myeloma; Cell proliferation; Apoptosis; Prognosis; Fluorescence in situ hybridization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100012 |
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| Nieddu,Mariella; Mezzanotte,Roberto; Pichiri,Giuseppina; Coni,Pier Paolo; Dedola,Gian Luca; Dettori,Maria Luisa; Pazzola,Michele; Vacca,Giuseppe Massimo; Robledo,Renato. |
| Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovidae; Satellite DNA; Fluorescence in situ hybridization; Pericentromeric region. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
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